Figure 1: Treatment of HUVE cells with VEGF stimulates phosphorylation of VEGFR-2 at Tyr1175, detected by PathScan™ Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA kit, #V2110-15P, but does not affect the level of total VEGFR-2 protein detected by PathScan™ Total VEGFR-2 Sandwich ELISA kit, #V2110-15M. OD450 readings are shown in the top figure, while the corresponding Western blot using Phospho-VEGFR-2 (Tyr1175) Rabbit Monoclonal Ab #2478 (right panel) or VEGFR-2 Rabbit Monoclonal Antibody (7340-55B11) (left panel), is shown in the bottom figure.
Figure 2: Linear relationship between protein concentration of lysates from VEGF-treated HUVE cells and kit assay optical density readings. HUVE cells (85% confluence) were treated with VEGF (50 ng/ml), and lysed after growth at 37oC for 2 min.
Supplier Page from United States Biological for Vascular Endothelial Growth Factor Receptor 2 ELISA Kit, BioAssay™ (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)